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er enrichment kit  (Invent Biotechnologies)


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    Structured Review

    Invent Biotechnologies er enrichment kit
    Er Enrichment Kit, supplied by Invent Biotechnologies, used in various techniques. Bioz Stars score: 95/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er/MinuteTM+ER+Enrichment+Kit/pm42887501-437-7-15
    Average 95 stars, based on 48 article reviews
    er enrichment kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Isolation:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: .. The ER was isolated using the Minute ER Enrichment Kit for Tissue and Cultured Cells (Invent Biotech). ..

    Article Title: Nur77 attenuates Paneth cell necroptosis-induced intestinal inflammation through regulating endoplasmic reticulum homeostasis in sepsis
    Article Snippet: Nuclear and cytoplasmic protein isolation of crypt cells was performed using Nuclear and Cytoplasmic Protein Extraction Kit (P0027, Beyotime) according to the manufacturer’s instructions. .. ER protein isolation of crypt cells was performed using Minute TM ER Enrichment Kit for Tissues and Cultured Cells (ER-036, Invent Biotechnologies) according to the manufacturer’s instructions. .. The amino acid sequences of Nur77, AMFR, and PKCα were acquired from the National Center for Biotechnology Information.

    Tissue:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: .. The ER was isolated using the Minute ER Enrichment Kit for Tissue and Cultured Cells (Invent Biotech). ..

    Cell Culture:

    Article Title: Targeting MMGT1 enhances immunotherapy efficacy in triple-negative breast cancer.
    Article Snippet: .. The ER was isolated using the Minute ER Enrichment Kit for Tissue and Cultured Cells (Invent Biotech). ..

    Article Title: Nur77 attenuates Paneth cell necroptosis-induced intestinal inflammation through regulating endoplasmic reticulum homeostasis in sepsis
    Article Snippet: Nuclear and cytoplasmic protein isolation of crypt cells was performed using Nuclear and Cytoplasmic Protein Extraction Kit (P0027, Beyotime) according to the manufacturer’s instructions. .. ER protein isolation of crypt cells was performed using Minute TM ER Enrichment Kit for Tissues and Cultured Cells (ER-036, Invent Biotechnologies) according to the manufacturer’s instructions. .. The amino acid sequences of Nur77, AMFR, and PKCα were acquired from the National Center for Biotechnology Information.

    other:

    Article Title: A novel MMP13 frameshift variant causes short stature via enhanced MMP13–HSPA5 interaction and activated endoplasmic reticulum stress
    Article Snippet: The ER Enrichment Kit (Invent Biotechnologies) was employed to isolate ER according to the manufacturer's instructions.

    Incubation:

    Article Title: Pyramiding elite alleles of the genetically linked OsNRAMP5 and OsHMA3 confers low Cd accumulation in rice grains without compromising stress tolerance
    Article Snippet: Vector combinations of pro35S:OsNRAMP5-GFP and pro35S:OsVAP1-3-3FLAG , or pro35S:GFP and pro35S:OsVAP1-3-3FLAG , were co-transformed into rice protoplasts using the polyethylene glycol-mediated method. .. After 12 h of incubation, ER proteins were extracted from rice protoplasts using the Minute Plant ER Enrichment Kit (Invent Biotechnologies, USA) and immunoprecipitated with anti-GFP immunomagnetic beads according to a previously described method ( ). .. Samples were subjected to immunoblot analysis using anti-GFP (Abclonal, USA) and anti-FLAG (Abclonal, USA) antibodies. pro35S :GFP coexpressed with OsVAP1-3-3FLAG was used as a negative control.

    Article Title: Pyramiding elite alleles of genetically linked OsNRAMP5 and OsHMA3 confers low-Cd grains without compromising stress tolerance in rice.
    Article Snippet: .. After incubation for 12 h, ER proteins were extracted from the rice 971 Jo urn al Pr -pr oo f 30 protoplasts using MinuteTM Plant ER Enrichment Kit (Invent Biotechnologies, USA) 972 and immunoprecipitated with anti-GFP immunomagnetic beads according to a 973 previously described method (Liu et al., 2024). .. The samples were then subjected to 974 immunoblotting analysis using anti-GFP (Abclonal, USA) and anti-FLAG (Abclonal, 975 USA) antibodies.

    Immunoprecipitation:

    Article Title: Pyramiding elite alleles of the genetically linked OsNRAMP5 and OsHMA3 confers low Cd accumulation in rice grains without compromising stress tolerance
    Article Snippet: Vector combinations of pro35S:OsNRAMP5-GFP and pro35S:OsVAP1-3-3FLAG , or pro35S:GFP and pro35S:OsVAP1-3-3FLAG , were co-transformed into rice protoplasts using the polyethylene glycol-mediated method. .. After 12 h of incubation, ER proteins were extracted from rice protoplasts using the Minute Plant ER Enrichment Kit (Invent Biotechnologies, USA) and immunoprecipitated with anti-GFP immunomagnetic beads according to a previously described method ( ). .. Samples were subjected to immunoblot analysis using anti-GFP (Abclonal, USA) and anti-FLAG (Abclonal, USA) antibodies. pro35S :GFP coexpressed with OsVAP1-3-3FLAG was used as a negative control.

    Article Title: Pyramiding elite alleles of genetically linked OsNRAMP5 and OsHMA3 confers low-Cd grains without compromising stress tolerance in rice.
    Article Snippet: .. After incubation for 12 h, ER proteins were extracted from the rice 971 Jo urn al Pr -pr oo f 30 protoplasts using MinuteTM Plant ER Enrichment Kit (Invent Biotechnologies, USA) 972 and immunoprecipitated with anti-GFP immunomagnetic beads according to a 973 previously described method (Liu et al., 2024). .. The samples were then subjected to 974 immunoblotting analysis using anti-GFP (Abclonal, USA) and anti-FLAG (Abclonal, 975 USA) antibodies.



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    Image Search Results


    Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and ER+ MCF-7 cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.

    Journal: Journal of Clinical Practice and Research

    Article Title: FOXM1 Drives Subtype-Specific Dysregulation of Prognostic miRNAs in Breast Cancer Cells

    doi: 10.14744/cpr.2026.18248

    Figure Lengend Snippet: Endogenous expression levels of the selected miRNAs in breast cancer and normal cells. The expression levels of miRNAs were analyzed by RT-qPCR in normal breast epithelial cells (MCF10A), ER− MDA-MB-231 cells, and ER+ MCF-7 cells. P<0.05; *P<0.01; **P<0.001; ***P<0.0001.

    Article Snippet: Human triple-negative breast cancer (MDA-MB-231) and estrogen receptor-positive (ER+) breast cancer (MCF-7) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Expressing, Quantitative RT-PCR

    Differential expression of miRNAs in response to FOXM1 knockdown. (a) Endogenous expression levels of seven miRNAs and transcriptionally active FOXM1 isoforms in ER− and ER+ cells. (b) miRNA expression levels were analyzed by RT-qPCR in MDA-MB-231 and MCF-7 cells. U6 snRNA was used as an internal control to normalize the expression data. Results are presented as the mean±SD of three independent experiments. P<0.05; *P<0.01; **P<0.001; ***p<0.0001.

    Journal: Journal of Clinical Practice and Research

    Article Title: FOXM1 Drives Subtype-Specific Dysregulation of Prognostic miRNAs in Breast Cancer Cells

    doi: 10.14744/cpr.2026.18248

    Figure Lengend Snippet: Differential expression of miRNAs in response to FOXM1 knockdown. (a) Endogenous expression levels of seven miRNAs and transcriptionally active FOXM1 isoforms in ER− and ER+ cells. (b) miRNA expression levels were analyzed by RT-qPCR in MDA-MB-231 and MCF-7 cells. U6 snRNA was used as an internal control to normalize the expression data. Results are presented as the mean±SD of three independent experiments. P<0.05; *P<0.01; **P<0.001; ***p<0.0001.

    Article Snippet: Human triple-negative breast cancer (MDA-MB-231) and estrogen receptor-positive (ER+) breast cancer (MCF-7) cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

    Techniques: Quantitative Proteomics, Knockdown, Expressing, Quantitative RT-PCR, Control